Negative autoregulation and bursty gene expression
A gene that represses its own transcription and makes its protein in bursts.
- Name:
negative_autoregulation - Field:
biology/systems_biology - Formalism: jump process
- References: Thattai and van Oudenaarden 2001; Becskei and Serrano 2000; arXiv:1608.06582
Factory
@register_model(
field='biology/systems_biology',
tags=('gene regulation', 'noise', 'bursts', 'jump process'),
references=('Thattai and van Oudenaarden 2001', 'Becskei and Serrano 2000', 'arXiv:1608.06582'),
defaults={'until': 200.0, 'dt': 1.0},
)
def negative_autoregulation(
p: float = 0.0,
*,
alpha: float = 2.0,
K: float = 50.0,
n: float = 1.0,
b: float = 10.0,
beta: float = 0.1,
delta: float = 1.0,
stochastic: bool = True,
) -> System:
"""Return a gene that represses its own transcription and makes its protein in bursts.
Every mRNA molecule yields on average `b / delta` proteins before it decays, so with fast mRNA decay the protein
arrives in geometric bursts and its count fluctuates far more than a Poisson count. Negative feedback lowers the
mean and the noise. A very large `K` removes the feedback.
Args:
p:
The initial protein count, with no mRNA.
alpha:
The transcription rate without repressor.
K:
The protein count at half repression.
n:
The Hill coefficient of repression.
b:
The translation rate per mRNA molecule.
beta:
The decay rate of the protein.
delta:
The decay rate of mRNA.
stochastic:
Whether the model is a jump process.
Returns:
system:
The model, with the counts `gene.m` and `gene.p`.
Examples:
>>> from skcomplex.models.biology.systems_biology import negative_autoregulation
>>> negative_autoregulation().interactions[0].modifiers
{('gene', 'p'): 1}
"""
mode = 'jump' if stochastic else 'rate'
feedback = _repress('autoregulation', 'gene', 'gene', {'alpha': alpha, 'alpha0': 0.0, 'K': K, 'n': n}, mode)
expression = _express('gene', b, beta, delta, mode)
return System(elements=[GeneElement('gene', p=p)], interactions=[feedback, *expression])
Components
class GeneElement(Element):
"""Gene with a count `m` of messenger RNA and a count `p` of protein.
A gene has no laws of its own. Reactions transcribe, translate, and degrade its molecules.
Args:
label:
The label of the gene.
visual:
How the element is drawn, or `None` for `default_visual_`.
m:
The initial number of mRNA molecules.
p:
The initial number of protein molecules.
Examples:
>>> from skcomplex.models.biology.systems_biology import GeneElement
>>> GeneElement('lacI', p=20.0).p
20.0
"""
def __init__(
self,
label: str,
visual: VisualSpec | None = None,
*,
m: Annotated[float, StateSpec(domain=RealSet.closed_open(0, oo))] = 0.0,
p: Annotated[float, StateSpec(domain=RealSet.closed_open(0, oo))] = 0.0,
) -> None:
super().__init__(label, visual=visual)
self.m = m
self.p = p
class RepressionInteraction(ReactionInteraction):
"""Synthesis of a product at the rate `alpha0 + alpha (1 - hill(y, K, n))`, repressed by a modifier `y`.
The product, such as the mRNA of a gene, gains units at the leaky rate `alpha0` plus the rate `alpha` lowered by the
decreasing Hill function `1 / (1 + (y / K)^n)` of the one modifier, the repressor. The repressor enters the rate
and does not change. In the mode `'jump'` every synthesis is a random event that adds one unit.
Args:
label:
The label of the repression.
reactants:
The coefficients of the reactants, or `None` for a synthesis from a source.
products:
The coefficient of the synthesized state, such as `{('gene', 'm'): 1}`.
modifiers:
The coefficient of the repressor state, such as `{('repressor', 'p'): 1}`.
alpha:
The synthesis rate without repressor.
alpha0:
The leaky synthesis rate at saturating repressor.
K:
The repressor amount at half repression.
n:
The Hill coefficient of the repression.
mode:
The form of the laws, `'rate'`, `'jump'`, or `'langevin'`.
Raises:
ValidationError:
The mode is unknown, the reaction has neither reactants nor products, or an entry is not a pair of a path
and a state name with a positive coefficient.
Examples:
>>> from skcomplex.core.system import System
>>> from skcomplex.engine.runtime import Simulation
>>> from skcomplex.models.biology.systems_biology import RepressionInteraction, SpeciesElement
>>> repression = RepressionInteraction(
... 'repression', products={('mRNA', 'x'): 1}, modifiers={('repressor', 'x'): 1}, alpha=2.0, K=1.0
... )
>>> species = [SpeciesElement('mRNA'), SpeciesElement('repressor', x=1.0)]
>>> trajectory = Simulation(System(elements=species, interactions=[repression]), dt=0.1).check().prepare()
>>> round(float(trajectory.run(until=1.0).trajectory_.values('mRNA.x')[-1]), 3)
1.0
"""
def __init__(
self,
label: str,
*,
reactants: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
products: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
modifiers: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
alpha: Annotated[float, ParameterSpec(domain=RealSet.closed_open(0, oo))] = 1.0,
alpha0: Annotated[float, ParameterSpec(domain=RealSet.closed_open(0, oo))] = 0.0,
K: Annotated[float, ParameterSpec(domain=RealSet.open(0, oo))] = 1.0,
n: Annotated[float, ParameterSpec(domain=RealSet.open(0, oo))] = 1.0,
mode: str = 'rate',
) -> None:
super().__init__(label, reactants=reactants, products=products, modifiers=modifiers, mode=mode)
self.alpha = alpha
self.alpha0 = alpha0
self.K = K
self.n = n
def _get_rate(self, namespace: SimpleNamespace) -> Expression:
"""Return the repressed synthesis rate `alpha0 + alpha (1 - hill(y, K, n))` of the repressor `y`.
Args:
namespace:
The namespace of the symbols the rate can use, with the namespaces of the modifiers.
Returns:
rate:
The propensity of the synthesis.
"""
s = namespace
((repressor, _),) = self._get_terms(self._get_modifiers(), s, 'modifiers')
return s.alpha0 + s.alpha * (1 - hill(repressor, s.K, s.n))
class ReactionInteraction(JumpInteraction):
"""Reaction that moves units from its reactants to its products at a mass-action or a given propensity.
A reactant, a product, or a modifier is a pair of the path of an element, or of a member of a group such as
`cells[0]`, and the name of its state that holds the units, mapped to its stoichiometric coefficient. A coefficient
is a positive number, or a function of the namespace of the reaction that returns an expression of its parameters,
such as `lambda s: 1 / s.target.epsilon`. So `S + I -> 2 I` has the reactants `{('S', 'n'): 1, ('I', 'n'): 1}` and
the products `{('I', 'n'): 2}`, and two states of one element are `('cell', 'u')` and `('cell', 'v')`. A species may
also be the name of a state of the `target` alone, such as `{'u': 1, 'v': 2}`. A target that is a group or a field
reacts in every member, with the species of every member its own states, as the reactions at every site of a
reaction-diffusion field.
A subclass may bind its species under roles of its own names, such as `susceptible` and `infected`: it returns its
entries from `_get_reactants`, `_get_products`, and `_get_modifiers`, pairs of the path a role binds and a state
name, and the reaction reads every pair from the namespace of the role that binds its path.
The propensity is `k` times the product of every reactant and modifier state to the power of its coefficient, or the
expression the `propensity` function returns. The reaction changes every state by its coefficient among the products
minus its coefficient among the reactants, so it conserves the total of the states whose changes sum to zero, and
modifiers enter the propensity but do not change.
In the mode `'rate'` the reaction is a rate equation. In the mode `'jump'` it is the random event of a
`JumpInteraction` at the propensity that moves the units of the coefficients. In the mode `'langevin'` it is the
chemical Langevin equation, the rate equation with one Wiener process of amplitude the square root of the propensity
driving every state the reaction changes.
In the `'agent'` form the target is an agent group and every member reacts on its own states, the species named by
state names alone, in every step from the states at its start. The propensity of a member is `k` times the product
of its reactant and modifier states to the power of their coefficients, or the number `propensity(agent, model)`
returns, and a coefficient that is a function is the number `coefficient(agent, model)` returns. In the mode
`'rate'` the member adds the changes times its propensity, one unit step of the rate equation, and in the mode
`'jump'` it adds the changes with the probability `1 - exp(-propensity)` that an event fires in the step. The
members react at once from the states at the start of the rule under the schedule `'synchronous'`, one after the
other in their order or in a new random order every step under `'sequential'` or `'random-sequential'`, or each in
its own activation under `'member'`.
Args:
label:
The label of the reaction, unique among the parts of its system.
target:
The path of the element, group, or field whose states the species named by a state alone are, or `None`.
reactants:
The coefficients of the reactants, or `None` for a source.
products:
The coefficients of the products, or `None` for a sink.
k:
The rate constant.
propensity:
A function of the namespace of the reaction that returns the propensity, or `None` for mass action. The
namespace holds the tuples `reactants`, `products`, and `modifiers` of the namespaces of the elements their
pairs bind, in the order of their entries, `target`, the namespace of the target or of one of its members,
and the symbol `k`. In the agent form, a function `propensity(agent, model)` that returns a number.
modifiers:
The coefficients of the modifiers, whose states the propensity reads but the reaction does not change, or
`None`.
mode:
The form of the laws, `'rate'`, `'jump'`, or `'langevin'`.
form:
`'symbolic'` for the laws of the states, or `'agent'` for a rule in which every member of an agent group
reacts on its own states.
phase:
The phase of the step the agent form runs in, or `None` for the default phase.
schedule:
The order of the members in the agent form, `'synchronous'`, `'sequential'`, `'random-sequential'`, or
`'member'`.
Raises:
ValidationError:
The mode, the form, or the schedule is unknown, the symbolic form is not synchronous, the reaction has
neither reactants nor products, an entry is neither a pair of a path and a state name nor a state name of a
given target, a coefficient is neither a positive number nor a function, or the agent form has no target,
has an entry that is a pair, or has the mode `'langevin'`.
Examples:
>>> from skcomplex.core.elements import CompartmentElement
>>> from skcomplex.core.interactions import ReactionInteraction
>>> from skcomplex.core.system import System
>>> from skcomplex.engine.runtime import Simulation
>>> infection = ReactionInteraction(
... 'infection', reactants={('S', 'n'): 1, ('I', 'n'): 1}, products={('I', 'n'): 2}, k=0.0005
... )
>>> recovery = ReactionInteraction('recovery', reactants={('I', 'n'): 1}, products={('R', 'n'): 1}, k=0.1)
>>> system = System(
... elements=[CompartmentElement('S', n=990), CompartmentElement('I', n=10), CompartmentElement('R')],
... interactions=[infection, recovery],
... )
>>> states = Simulation(system, dt=1.0).check().prepare().run(until=50).trajectory_.states
>>> round(float(states[-1].sum()), 6)
1000.0
The decay `n -> 0` at every site of a field, at the rate `k n` of the site:
>>> import numpy as np
>>> from skcomplex.core.elements import FieldElement
>>> from skcomplex.core.spaces import LatticeSpace
>>> decay = ReactionInteraction('decay', target='cells', reactants={'n': 1}, k=1.0)
>>> values = {'n': np.arange(3.0)}
>>> cells = FieldElement('cells', element=CompartmentElement, space=LatticeSpace((3,)), values=values)
>>> simulation = Simulation(System(elements=[cells], interactions=[decay]), dt=1.0).check().prepare()
>>> np.round(simulation.run(until=1).trajectory_.lattice('cells.n')[-1], 3).tolist()
[0.0, 0.368, 0.736]
A coefficient that is a parameter, the decay at the rate `x` that removes `1 / epsilon` units per event:
>>> from typing import Annotated
>>> from skcomplex.core.elements import Element
>>> from skcomplex.specs import ParameterSpec, StateSpec
>>> class ScaledElement(Element):
... def __init__(
... self,
... label,
... x: Annotated[float, StateSpec()] = 1.0,
... epsilon: Annotated[float, ParameterSpec()] = 0.5,
... ):
... super().__init__(label)
... self.x = x
... self.epsilon = epsilon
>>> scaled = ReactionInteraction(
... 'decay', target='box', reactants={'x': lambda s: 1 / s.target.epsilon}, propensity=lambda s: s.target.x
... )
>>> simulation = Simulation(System(elements=[ScaledElement('box')], interactions=[scaled]), dt=0.01)
>>> round(float(simulation.check().prepare().run(until=0.5).trajectory_.values('box.x')[-1]), 3)
0.368
Every agent of a group decays on its own states, by `k n` in a step of the rate equation:
>>> from skcomplex.core.elements import GroupElement
>>> from skcomplex.core.spaces import DiscreteTime
>>> class CellElement(Element):
... def __init__(self, label, n: Annotated[float, StateSpec()] = 3.0):
... super().__init__(label)
... self.n = n
>>> cells = GroupElement('cells', element=CellElement, size=2)
>>> decay = ReactionInteraction('decay', target='cells', reactants={'n': 1}, k=0.1, form='agent')
>>> system = System(elements=[cells], interactions=[decay], time=DiscreteTime())
>>> trajectory = Simulation(system).check().prepare().run(until=2).trajectory_
>>> np.round(trajectory.agents('cells', 2).values['n'], 6).tolist()
[2.43, 2.43]
"""
def __init__(
self,
label: str,
*,
target: Annotated[str | None, RoleSpec()] = None,
reactants: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
products: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
k: Annotated[float, ParameterSpec(domain=RealSet.closed_open(0, oo))] = 1.0,
propensity: Callable[..., object] | None = None,
modifiers: Annotated[Stoichiometry | None, RoleSpec(variadic=True)] = None,
mode: str = 'rate',
form: str = 'symbolic',
phase: str | None = None,
schedule: str = 'synchronous',
) -> None:
super().__init__(label, target='')
self.target = target
self.reactants = reactants
self.products = products
self.k = k
self.propensity = propensity
self.modifiers = modifiers
self.mode = mode
self.form = form
self.phase = phase
self.schedule = schedule
def _get_reactants(self) -> Stoichiometry | None:
"""Return the coefficients of the reactants, which a subclass with roles of its own names returns."""
return self.reactants
def _get_products(self) -> Stoichiometry | None:
"""Return the coefficients of the products, which a subclass with roles of its own names returns."""
return self.products
def _get_modifiers(self) -> Stoichiometry | None:
"""Return the coefficients of the modifiers, which a subclass with roles of its own names returns."""
return self.modifiers
def _get_mode(self) -> str:
"""Return the form of the laws, which a subclass that sets it by another argument returns."""
return self.mode
def _get_bound(self, namespace: SimpleNamespace) -> dict[str, SimpleNamespace]:
"""Return the namespace of every path the plain roles of the reaction bind, by path."""
roles = [
name
for name, spec in self._get_specs().items()
if isinstance(spec, RoleSpec) and not spec.variadic and not spec.noise
]
return {
getattr(self, name): getattr(namespace, name)
for name in roles
if isinstance(getattr(self, name), str) and hasattr(namespace, name)
}
def _get_terms(
self,
entries: Stoichiometry | None,
namespace: SimpleNamespace,
name: str,
) -> list[tuple[Expression, Expression | float]]:
"""Return the state symbol and the coefficient of every entry of a kind of species.
A state name alone is a state of the target. A pair reads the namespace of the plain role that binds its path,
or else the namespace of its variadic role `name`, in the order of the pairs. A coefficient that is a function
is its expression at the namespace.
"""
entries = entries or {}
pairs = [entry for entry in entries if not isinstance(entry, str)]
sources: dict[object, SimpleNamespace] = {}
if pairs and name in self._get_specs():
sources = dict(zip(pairs, getattr(namespace, name), strict=True))
elif pairs:
bound = self._get_bound(namespace)
sources = {pair: bound[pair[0]] for pair in pairs}
return [
(
getattr(
self._get_member(namespace, 'target') if isinstance(entry, str) else sources[entry],
self._get_state(entry),
),
SR(coefficient(namespace)) if callable(coefficient) else coefficient,
)
for entry, coefficient in entries.items()
]
def _get_state(self, entry: tuple[str, str] | str) -> str:
"""Return the name of the state of an entry, a state name alone or the second item of a pair."""
return entry if isinstance(entry, str) else entry[1]
def _get_changes(self, namespace: SimpleNamespace) -> dict[Expression, float]:
"""Return the net change of every state the reaction changes, products minus reactants."""
changes: dict[Expression, float] = {}
for sign, entries, name in (
(-1, self._get_reactants(), 'reactants'),
(1, self._get_products(), 'products'),
):
for state, coefficient in self._get_terms(entries, namespace, name):
changes[state] = changes.get(state, 0) + sign * coefficient
return {state: change for state, change in changes.items() if change}
def _get_mass_action(self, namespace: SimpleNamespace, constant: Expression) -> Expression:
"""Return the mass action of a rate constant, times every reactant and modifier to its coefficient."""
propensity = SR(constant)
for entries, name in ((self._get_reactants(), 'reactants'), (self._get_modifiers(), 'modifiers')):
for state, coefficient in self._get_terms(entries, namespace, name):
propensity *= state**coefficient
return propensity
def _get_rate(self, namespace: SimpleNamespace) -> Expression:
"""Return the propensity of the reaction, by the user's function or by mass action at the rate constant `k`."""
if self.propensity is not None:
return SR(self.propensity(namespace))
return self._get_mass_action(namespace, self._get_parameter_symbol('k', namespace))
def _get_langevin_laws(self, propensity: Expression, changes: dict[Expression, float]) -> list[BasePart]:
"""Return the drift of the reaction and the noise of its one Wiener process on every state it changes."""
amplitude = sqrt(where(propensity > 0, propensity, 0))
rates: list[BasePart] = [RateLaw(state, change * propensity) for state, change in changes.items()]
noises: list[BasePart] = [
NoiseLaw(state, change * amplitude, process=self.label) for state, change in changes.items()
]
return rates + noises
def _is_entry(self, entry: object) -> bool:
"""Check whether an entry is a pair of a path and a state name, or a state name of a given target."""
if isinstance(entry, str):
return self.target is not None and entry.isidentifier()
return (
isinstance(entry, tuple)
and len(entry) == ENTRY_SIZE
and all(isinstance(item, str) for item in entry)
and entry[1].isidentifier()
)
def _is_coefficient(self, coefficient: object) -> bool:
"""Check whether a coefficient is a positive number or a function."""
return callable(coefficient) or (isinstance(coefficient, int | float) and coefficient > 0)
def _check_entries(self, name: str, entries: Stoichiometry | None) -> None:
"""Raise an error for entries that are neither pairs nor state names of the target, or not coefficients."""
if entries is None:
return
valid = isinstance(entries, Mapping) and all(
self._is_entry(entry) and self._is_coefficient(coefficient) for entry, coefficient in entries.items()
)
if not valid:
error_msg = (
f'The {name} of reaction {self.label!r} are {entries!r}. Map pairs of a path and a state name, or '
'state names of the target, to positive numbers or functions, such as {("S", "n"): 1} or {"n": 1}.'
)
raise ValidationError(error_msg)
def _check_form(self) -> None:
"""Raise an error for an unknown form, or an agent form without a target, with pairs, or of Langevin mode."""
if self.form not in FORMS:
error_msg = f'Reaction {self.label!r} has the form {self.form!r}. Use one of {", ".join(FORMS)}.'
raise ValidationError(error_msg)
self._check_variant('schedule', self.schedule, SCHEDULES)
if self.form != 'agent' and self.schedule != 'synchronous':
error_msg = f'The schedule {self.schedule!r} of reaction {self.label!r} runs agent rules. Use form="agent".'
raise ValidationError(error_msg)
if self.form != 'agent':
return
if self.target is None:
error_msg = f'Reaction {self.label!r} has the agent form but no target. Give the agent group as `target`.'
raise ValidationError(error_msg)
mode = self._get_mode()
if mode not in AGENT_MODES:
error_msg = (
f'Reaction {self.label!r} has the agent form and the mode {mode!r}. Use {" or ".join(AGENT_MODES)}.'
)
raise ValidationError(error_msg)
entries = [*(self._get_reactants() or {}), *(self._get_products() or {}), *(self._get_modifiers() or {})]
if not all(isinstance(entry, str) for entry in entries):
error_msg = (
f'Reaction {self.label!r} has the agent form, whose species are states of every member. Name them by '
'state names alone, such as {"n": 1}.'
)
raise ValidationError(error_msg)
def _check_jump(self) -> None:
"""Raise an error for an unknown mode or form, no reactants and products, or entries the reaction cannot take.
Raises:
ValidationError:
The mode or the form is unknown, the reaction has neither reactants nor products, an entry is neither a
pair of a path and a state name nor a state name of a given target, a coefficient is neither a positive
number nor a function, or the agent form has no target, has an entry that is a pair, or has the mode
`'langevin'`.
"""
mode = self._get_mode()
if mode not in MODES:
error_msg = f'Reaction {self.label!r} has the mode {mode!r}. Use one of {", ".join(MODES)}.'
raise ValidationError(error_msg)
reactants, products = self._get_reactants(), self._get_products()
if not reactants and not products:
error_msg = f'Reaction {self.label!r} has neither reactants nor products. Give at least one of them.'
raise ValidationError(error_msg)
for name, entries in (('reactants', reactants), ('products', products), ('modifiers', self._get_modifiers())):
self._check_entries(name, entries)
self._check_form()
def _get_number(self, value: object, agent: AgentViewProtocol, model: ModelViewProtocol) -> float:
"""Return a number, or the value of a function of an agent and the model as a number."""
found = value(agent, model) if callable(value) else value
if not isinstance(found, SupportsFloat):
error_msg = f'A function of reaction {self.label!r} returns {found!r} for an agent. Return a number.'
raise ValidationError(error_msg)
return float(found)
def _get_agent_changes(self, agent: AgentViewProtocol, model: ModelViewProtocol) -> dict[str, float]:
"""Return the net change of every state of an agent the reaction changes, products minus reactants."""
changes: dict[str, float] = {}
for sign, entries in ((-1, self._get_reactants()), (1, self._get_products())):
for entry, coefficient in (entries or {}).items():
state = self._get_state(entry)
changes[state] = changes.get(state, 0.0) + sign * self._get_number(coefficient, agent, model)
return changes
def _get_agent_propensity(self, agent: AgentViewProtocol, model: ModelViewProtocol) -> float:
"""Return the propensity of an agent, by the user's function or by mass action at the rate constant `k`."""
if self.propensity is not None:
return self._get_number(self.propensity, agent, model)
propensity = self._get_parameter_value('k', model)
for entries in (self._get_reactants(), self._get_modifiers()):
for entry, coefficient in (entries or {}).items():
value = float(getattr(agent, self._get_state(entry)))
propensity *= value ** self._get_number(coefficient, agent, model)
return propensity
def _get_agent_scale(self, propensity: float, model: ModelViewProtocol) -> float:
"""Return the multiple of its changes an agent adds in a step, its propensity or whether an event fires."""
if self._get_mode() == 'rate':
return propensity
return float(model.rng.random() < 1.0 - math.exp(-max(propensity, 0.0)))
def _get_agent_step(self, agent: AgentViewProtocol, model: ModelViewProtocol) -> dict[str, float]:
"""Return the change of every state of an agent in the step, its changes times its propensity or its event."""
changes, propensity = self._get_agent_changes(agent, model), self._get_agent_propensity(agent, model)
scale = self._get_agent_scale(propensity, model)
return {state: change * scale for state, change in changes.items()}
def _apply_agent_step(self, agent: AgentViewProtocol, changes: dict[str, float]) -> None:
"""Add their changes to the states of an agent."""
for state, change in changes.items():
setattr(agent, state, float(getattr(agent, state)) + change)
def _react_member(self, agent: AgentViewProtocol, model: ModelViewProtocol) -> None:
"""React in one agent on its own states, in its own activation."""
if agent.alive:
self._apply_agent_step(agent, self._get_agent_step(agent, model))
def react(self, model: ModelViewProtocol) -> None:
"""React in every agent of the target on its own states, by the schedule.
Args:
model:
The model view of the interaction.
"""
self._update_agents(model, self.schedule, self._get_agent_step, self._apply_agent_step)
def _get_laws(self, namespace: SimpleNamespace) -> list[BasePart]:
"""Return the laws of the reaction in its mode, or the rule of the agent form.
Args:
namespace:
The namespace of the symbols the laws can use, with the namespaces of the target, the reactants, the
products, and the modifiers.
Returns:
laws:
The rate laws, the jump law, the rate and noise laws, or the rule of the reaction.
"""
if self.form == 'agent' and self.schedule == 'member':
return [RuleLaw(self._react_member, phase=self.phase, members=self._get_role('target'))]
if self.form == 'agent':
return [RuleLaw(self.react, phase=self.phase)]
mode = self._get_mode()
if mode == 'jump':
return [*super()._get_laws(namespace)]
propensity = self._get_rate(namespace)
changes = self._get_changes(namespace)
if mode == 'langevin':
return self._get_langevin_laws(propensity, changes)
return [RateLaw(state, change * propensity) for state, change in changes.items()]